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Influence of high-pressure homogenization, ultrasonication, and supercritical fluid on free astaxanthin extraction from β-glucanase-treated Phaffia rhodozyma cells.
- Source :
- Preparative Biochemistry & Biotechnology; 2016, Vol. 46 Issue 2, p116-122, 7p, 1 Diagram, 6 Graphs
- Publication Year :
- 2016
-
Abstract
- In this study astaxanthin production byPhaffia rhodozymawas enhanced by chemical mutation using ethyl methane sulfonate. The mutant produces a higher amount of astaxanthin than the wild yeast strain. In comparison to supercritical fluid technique, high-pressure homogenization is better for extracting astaxanthin from yeast cells. Ultrasonication of dimethyl sulfoxide, hexane, and acetone-treated cells yielded less astaxanthin than β-glucanase enzyme-treated cells. The combination of ultrasonication with β-glucanase enzyme is found to be the most efficient method of extraction among all the tested physical and chemical extraction methods. It gives a maximum yield of 435.71 ± 6.55 µg free astaxanthin per gram of yeast cell mass. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 10826068
- Volume :
- 46
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Preparative Biochemistry & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 112194398
- Full Text :
- https://doi.org/10.1080/10826068.2014.995807