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T Cell Receptor Mediated Calcium Entry Requires Alternatively Spliced Cav1.1 Channels.
- Source :
- PLoS ONE; 1/27/2016, Vol. 11 Issue 1, p1-17, 17p
- Publication Year :
- 2016
-
Abstract
- The process of calcium entry in T cells is a multichannel and multi-step process. We have studied the requirement for L-type calcium channels (Ca<subscript>v</subscript>1.1) α1S subunits during calcium entry after TCR stimulation. High expression levels of Ca<subscript>v</subscript>1.1 channels were detected in activated T cells. Sequencing and cloning of Ca<subscript>v</subscript>1.1 channel cDNA from T cells revealed that a single splice variant is expressed. This variant lacks exon 29, which encodes the linker region adjacent to the voltage sensor, but contains five new N-terminal exons that substitute for exons 1 and 2, which are found in the Ca<subscript>v</subscript>1.1 muscle counterpart. Overexpression studies using cloned T cell Ca<subscript>v</subscript>1.1 in 293HEK cells (that lack TCR) suggest that the gating of these channels was altered. Knockdown of Ca<subscript>v</subscript>1.1 channels in T cells abrogated calcium entry after TCR stimulation, suggesting that Ca<subscript>v</subscript>1.1 channels are controlled by TCR signaling. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cell receptors
CALCIUM
CALCIUM channels
MOLECULAR cloning
GENETIC engineering
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 112553892
- Full Text :
- https://doi.org/10.1371/journal.pone.0147379