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Urban riverine environment is a source of multidrug-resistant and ESBL-producing clinically important Acinetobacter spp.

Authors :
Maravić, Ana
Skočibušić, Mirjana
Fredotović, Željana
Šamanić, Ivica
Cvjetan, Svjetlana
Knezović, Mia
Puizina, Jasna
Source :
Environmental Science & Pollution Research; Feb2016, Vol. 23 Issue 4, p3525-3535, 11p
Publication Year :
2016

Abstract

Some Acinetobacter species have emerged as very important opportunistic pathogens in humans. We investigated Acinetobacter spp. from the polluted urban riverine environment in Croatia in regard to species affiliation, antibiotic resistance pattern, and resistance mechanisms. Considerable number of isolates produced acquired extended-spectrum β-lactamase(s) (ESBLs), CTX-M-15 solely or with TEM-116. By Southern blot hybridization, bla was identified on plasmids ca. 10, 3, and 1.2 kb in Acinetobacter junii, A. gandensis, and A. johnsonii. The bla-carrying plasmid in A. gandensis was successfully transferred by conjugation to azide-resistant Escherichia coli J53. A. radioresistens isolate also carried an intrinsic carbapenemase gene bla with IS Aba1 insertion sequence present upstream to promote its expression. Majority of ESBL-producing isolates harbored integrases intI1 and/or intI2 and the sulfamethoxazole resistance gene sul1. Almost all isolates had overexpressed resistance-nodulation-cell division (RND) efflux system, indicating that this mechanism may have contributed to multidrug resistance phenotypes. This is the first report of environmental CTX-M-15-producing Acinetobacter spp. and the first identification of CTX-M-15 in A. johnsonii, A. junii, A. calcoaceticus, A. gandensis, A. haemolyticus, and A. radioresistens worldwide. We identified, also for the first time, the environmental Acinetobacter-producing TEM ESBLs, highlighting the potential risk for human health, and the role of these bacteria in maintenance and dissemination of clinically important antibiotic resistance genes in community through riverine environments. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09441344
Volume :
23
Issue :
4
Database :
Complementary Index
Journal :
Environmental Science & Pollution Research
Publication Type :
Academic Journal
Accession number :
112690979
Full Text :
https://doi.org/10.1007/s11356-015-5586-0