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Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish ? common carp cross.

Authors :
Shaojun Liu
Jing Luo
Jing Chai
Li Ren
Yi Zhou
Feng Huang
Xiaochuan Liu
Yubao Chen
Chun Zhang
Min Tao
Bin Lu
Wei Zhou
Guoliang Lin
Chao Mai
Shuo Yuan
Jun Wang
Tao Li
Qinbo Qin
Hao Feng
Kaikun Luo
Source :
Proceedings of the National Academy of Sciences of the United States of America; 2/2/2016, Vol. 113 Issue 5, p1327-1332, 6p
Publication Year :
2016

Abstract

Polyploidy is much rarer in animals than in plants but it is not known why. The outcome of combining two genomes in vertebrates remains unpredictable, especially because polyploidization seldom shows positive effects and more often results in lethal consequences because viable gametes fail to form during meiosis. Fortunately, the goldfish (maternal) ? common carp (paternal) hybrids have reproduced successfully up to generation 22, and this hybrid lineage permits an investigation into the genomics of hybridization and tetraploidization. The first two generations of these hybrids are diploids, and subsequent generations are tetraploids. Liver transcriptomes from four generations and their progenitors reveal chimeric genes (>9%) and mutations of orthologous genes. Characterizations of 18 randomly chosen genes from genomic DNA and cDNA confirm the chimera. Some of the chimeric and differentially expressed genes relate to mutagenesis, repair, and cancer-related pathways in 2nF1. Erroneous DNA excision between homologous parental genes may drive the high percentage of chimeric genes, or even more potential mechanisms may result in this phenomenon. Meanwhile, diploid offspring show paternal-biased expression, yet tetraploids show maternal-biased expression. These discoveries reveal that fast and unstable changes are mainly deleterious at the level of transcriptomes although some offspring still survive their genomic abnormalities. In addition, the synthetic effect of genome shock might have resulted in greatly reduced viability of 2nF2 hybrid offspring. The goldfish ? common carp hybrids constitute an ideal system for unveiling the consequences of intergenomic interactions in hybrid vertebrate genomes and their fertility. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00278424
Volume :
113
Issue :
5
Database :
Complementary Index
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Academic Journal
Accession number :
112857520
Full Text :
https://doi.org/10.1073/pnas.1512955113