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Single-Cell Gene Expression Analyses Reveal Heterogeneous Responsiveness of Fetal Innate Lymphoid Progenitors to Notch Signaling.
- Source :
- Cell Reports; Feb2016, Vol. 14 Issue 6, p1500-1516, 17p
- Publication Year :
- 2016
-
Abstract
- Summary T and innate lymphoid cells (ILCs) share some aspects of their developmental programs. However, although Notch signaling is strictly required for T cell development, it is dispensable for fetal ILC development. Constitutive activation of Notch signaling, at the common lymphoid progenitor stage, drives T cell development and abrogates ILC development by preventing Id2 expression. By combining single-cell transcriptomics and clonal culture strategies, we characterize two heterogeneous α 4 β 7 -expressing lymphoid progenitor compartments. αLP1 (Flt3 + ) still retains T cell potential and comprises the global ILC progenitor, while αLP2 (Flt3 − ) consists of ILC precursors that are primed toward the different ILC lineages. Only a subset of αLP2 precursors is sensitive to Notch signaling required for their proliferation. Our study identifies, in a refined manner, the diversity of transitional stages of ILC development, their transcriptional signatures, and their differential dependence on Notch signaling. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 26391856
- Volume :
- 14
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Cell Reports
- Publication Type :
- Academic Journal
- Accession number :
- 112907295
- Full Text :
- https://doi.org/10.1016/j.celrep.2016.01.015