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Hierarchy Low CD4+/CD8+ T-Cell Counts and IFN-γ Responses in HIV-1+ Individuals Correlate with Active TB and/or M.tb Co-Infection.
- Source :
- PLoS ONE; 3/9/2016, Vol. 11 Issue 3, p1-12, 12p
- Publication Year :
- 2016
-
Abstract
- Objective: Detailed studies of correlation between HIV-M.tb co-infection and hierarchy declines of CD8+/CD4+ T-cell counts and IFN-γ responses have not been done. We conducted case-control studies to address this issue. Methods: 164 HIV-1-infected individuals comprised of HIV-1<superscript>+</superscript>ATB, HIV-1<superscript>+</superscript>LTB and HIV-1<superscript>+</superscript>TB<superscript>-</superscript> groups were evaluated. Immune phenotyping and complete blood count (CBC) were employed to measure CD4+ and CD8+ T-cell counts; T.SPOT.TB and intracellular cytokine staining (ICS) were utilized to detect ESAT6, CFP10 or PPD-specific IFN-γ responses. Results: There were significant differences in median CD4+ T-cell counts between HIV-1<superscript>+</superscript>ATB (164/μL), HIV-1<superscript>+</superscript>LTB (447/μL) and HIV-1<superscript>+</superscript>TB<superscript>-</superscript> (329/μL) groups. Hierarchy low CD4+ T-cell counts (<200/μL, 200-500/μL, >500/μL) were correlated significantly with active TB but not M.tb co-infection. Interestingly, hierarchy low CD8+ T-cell counts were not only associated significantly with active TB but also with M.tb co-infection (P<0.001). Immunologically, HIV-1<superscript>+</superscript>ATB group showed significantly lower numbers of ESAT-6-/CFP-10-specific IFN-γ+ T cells than HIV-1<superscript>+</superscript>LTB group. Consistently, PPD-specific IFN-γ+CD4+/CD8+ T effector cells in HIV-1<superscript>+</superscript>ATB group were significantly lower than those in HIV-1<superscript>+</superscript>LTB group (P<0.001). Conclusions: Hierarchy low CD8+ T-cell counts and effector function in HIV-1-infected individuals are correlated with both M.tb co-infection and active TB. Hierarchy low CD4+ T-cell counts and Th1 effector function in HIV-1+ individuals are associated with increased frequencies of active TB, but not M.tb co-infection. [ABSTRACT FROM AUTHOR]
- Subjects :
- CD4 antigen
CD8 antigen
T cells
PHENOTYPES
CYTOKINES
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 113610125
- Full Text :
- https://doi.org/10.1371/journal.pone.0150941