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MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum.
- Source :
- BioMed Research International; 12/31/2015, Vol. 2015, p1-7, 7p
- Publication Year :
- 2015
-
Abstract
- Myeloid differentiation primary response gene 88 (MYD88), a universal adapter protein, plays an important role in activating the nuclear factor-κB (NF-κB) and regulating the expression of proinflammatory genes like tumor necrosis factor (TNF) and interleukin-1 (IL-1), which were highly involved in Salmonella Pullorum infection. To detect the relationship between polymorphisms of the MyD88 gene and Salmonella Pullorum disease, we screened the coding region (CDS) of the MYD88 gene by DNA pool construction and sequencing based on case-control study. Eight single nucleotide polymorphisms (SNPs) in the sequenced fragment (5 exons), 7 known loci and one novel mutation named G4810372T (SNP8), were found in the fifth exon. In addition, we found 7 nonsynonymous substitutions. The allele frequency of only one SNP, g.4810191C > T (SNP1), was significantly different (P<0.05) between case and control groups. The genotype frequencies of SNP1 (g.4810191C > T) and SNP3 (g.4810257G > T) were of significant difference between the case and the control groups (P<0.05). Collectively, SNPs of the MyD88 gene were significantly associated with susceptibility to Salmonella Pullorum infection, which can be used as a disease-resistant marker in chicken. These results provided a theoretical basis for future research on chicken breeding by marker-assisted selection. [ABSTRACT FROM AUTHOR]
- Subjects :
- SALMONELLA diseases
GENES
ALLELES
ANIMAL experimentation
BIOMARKERS
CHI-squared test
CONFIDENCE intervals
DISEASE susceptibility
GENETIC polymorphisms
GENETIC mutation
POLYMERASE chain reaction
POULTRY
PROBABILITY theory
RESEARCH funding
SALMONELLA
CASE-control method
DATA analysis software
HAPLOTYPES
DESCRIPTIVE statistics
ODDS ratio
GENOTYPES
DISEASE risk factors
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2015
- Database :
- Complementary Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 113630273
- Full Text :
- https://doi.org/10.1155/2015/692973