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Schistosoma japonicum HSP60-derived peptide SJMHE1 suppresses delayed-type hypersensitivity in a murine model.
- Source :
- Parasites & Vectors; 3/12/2016, Vol. 9, p1-11, 11p
- Publication Year :
- 2016
-
Abstract
- Background: Parasite-derived molecules with immunomodulatory properties, which have been optimised during host-parasite co-evolution, exhibit potential applications as novel immunotherapeutics. We have previously demonstrated that Schistosoma japonicum HSP60-derived peptide SJMHE1 induces CD4<superscript>+</superscript>CD25<superscript>+</superscript> regulatory T-cells (Tregs) and that adoptively transferred SJMHE1-induced CD4<superscript>+</superscript>CD25<superscript>+</superscript> Tregs inhibit delayed-type hypersensitivity (DTH) in mice. However, multiple concerns regarding this method render this treatment unsuitable. To gain further insights into the potential effects of SJMHE1, we used ovalbumin (OVA)-induced DTH and evaluated the effect of SJMHE1 on DTH mice. Methods: BALB/c mice were sensitised with OVA alone or combined with SJMHE1 and then challenged with OVA to induce DTH. We first analysed the potential effects of SJMHE1 by measuring DTH responses, T-cell responses, cytokine secretion, and Treg proportions. We then evaluated the expression levels of IL-10 and TGF-β1 in CD4<superscript>+</superscript>CD25<superscript>+</superscript> T-cells during DTH and Treg generation to identify the mechanism by which SJMHE1 suppresses DTH. Results: SJMHE1 modulated the effector response against OVA-induced DTH and stimulated the production of the anti-inflammatory cytokines IL-10 and TGF-β1 in immunised mice through a mechanism involving CD4<superscript>+</superscript>CD25<superscript>+</superscript> Tregs. SJMHE1-induced CD4<superscript>+</superscript>CD25<superscript>+</superscript> Tregs expressed high levels of CTLA-4, IL-10, and TGF-β1, which substantially contributed to the suppressive activity during DTH. The administration of SJMHE1 to DTH in mice led to the expansion of CD4<superscript>+</superscript>CD25<superscript>+</superscript> Tregs from CD4<superscript>+</superscript>CD25- T-cells in the periphery, which inhibited DTH responses. Conclusions: Our study proves that the parasite-driven peptide suppresses DTH in mice, which may confer a new option for inflammation treatment. [ABSTRACT FROM AUTHOR]
- Subjects :
- SCHISTOSOMA japonicum
PEPTIDES
PARASITES
T cells
MICE
Subjects
Details
- Language :
- English
- ISSN :
- 17563305
- Volume :
- 9
- Database :
- Complementary Index
- Journal :
- Parasites & Vectors
- Publication Type :
- Academic Journal
- Accession number :
- 113741619
- Full Text :
- https://doi.org/10.1186/s13071-016-1434-4