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Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers.
- Source :
- Biomarkers; Dec2015, Vol. 20 Issue 8, p572-589, 18p
- Publication Year :
- 2015
-
Abstract
- Context: There is insufficient knowledge about the chondrocyte membranome and its molecular composition. Objective: To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins. Materials and methods: Articular chondrocytes from equine metacarpophalangeal joints were separated into hydrophobic and hydrophilic fractions; trypsin-digested proteins were analysed by nanoLC-MS/MS. Results: A total of 315 proteins were identified. The phase extraction method yielded a high proportion of membrane proteins (56%) including CD276, S100-A6 and three VDAC isoforms. Discussion: Defining the chondrocyte membranome is likely to reveal new biomarker targets for conventional and biological drug discovery. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1354750X
- Volume :
- 20
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Biomarkers
- Publication Type :
- Academic Journal
- Accession number :
- 113744539
- Full Text :
- https://doi.org/10.3109/1354750X.2015.1130191