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Symbiont-mediated RNA interference in insects.
- Source :
- Proceedings of the Royal Society B: Biological Sciences; 2/24/2016, Vol. 283 Issue 1825, p1-9, 9p, 1 Color Photograph, 2 Charts, 4 Graphs
- Publication Year :
- 2016
-
Abstract
- RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were horizontally transmissible. This represents a significant advance in the ability to deliver RNAi, potentially to a large range of non-model insects. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09628452
- Volume :
- 283
- Issue :
- 1825
- Database :
- Complementary Index
- Journal :
- Proceedings of the Royal Society B: Biological Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 113940840
- Full Text :
- https://doi.org/10.1098/rspb.2016.0042