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Reduced Number of Transitional and Naive B Cells in Addition to Decreased BAFF Levels in Response to the T Cell Independent Immunogen Pneumovax®23.
- Source :
- PLoS ONE; 3/31/2016, Vol. 11 Issue 3, p1-17, 17p
- Publication Year :
- 2016
-
Abstract
- Protective immunity against T cell independent (TI) antigens such as Streptococcus pneumoniae is characterized by antibody production of B cells induced by the combined activation of T cell independent type 1 and type 2 antigens in the absence of direct T cell help. In mice, the main players in TI immune responses have been well defined as marginal zone (MZ) B cells and B-1 cells. However, the existence of human equivalents to these B cell subsets and the nature of the human B cell compartment involved in the immune reaction remain elusive. We therefore analyzed the effect of a TI antigen on the B cell compartment through immunization of healthy individuals with the pneumococcal polysaccharide (PnPS)-based vaccine Pneumovax<superscript>®</superscript>23, and subsequent characterization of B cell subpopulations. Our data demonstrates a transient decrease of transitional and naïve B cells, with a concomitant increase of IgA<superscript>+</superscript> but not IgM<superscript>+</superscript> or IgG<superscript>+</superscript> memory B cells and a predominant generation of PnPS-specific IgA<superscript>+</superscript> producing plasma cells. No alterations could be detected in T cells, or proposed human B-1 and MZ B cell equivalents. Consistent with the idea of a TI immune response, antigen-specific memory responses could not be observed. Finally, BAFF, which is supposed to drive class switching to IgA, was unexpectedly found to be decreased in serum in response to Pneumovax<superscript>®</superscript>23. Our results demonstrate that a characteristic TI response induced by Pneumovax<superscript>®</superscript>23 is associated with distinct phenotypical and functional changes within the B cell compartment. Those modulations occur in the absence of any modulations of T cells and without the development of a specific memory response. [ABSTRACT FROM AUTHOR]
- Subjects :
- IMMUNOGENETICS
T cells
ANTIGEN analysis
B cells
IMMUNE response
CELL compartmentation
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 114141896
- Full Text :
- https://doi.org/10.1371/journal.pone.0152215