Chlorogenic acid protects d -galactose-induced liver and kidney injury via antioxidation and anti-inflammation effects in mice.

ContextOxidative stress and inflammation are implicated in the aging process and its related hepatic and renal function decline. Chlorogenic acid (CGA) is one of the most abundant polyphenol compounds in the human diet. Recently, CGA has shownin vivoandin vitroantioxidant properties. ObjectiveThe current study investigates the effects of protective effects of chlorogenic acid (CGA) ond-galactose-induced liver and kidney injury. Materials and methodsHepatic and renal injuries were induced in a mouse model by subcutaneously injection ofd-galactose (d-gal; 100 mg/kg) once a day for 8 consecutive weeks and orally administered simultaneously with CGA included in the food (200 mg/kg of diet). The liver and renal functions were examined. Histological analyses of liver and kidney were done by haematoxylin and eosin staining. The oxidative stress markers and pro-inflammatory cytokines in the liver and the kidney were measured. ResultsCGA significantly reduced the serum aminotransferase, serum creatinine (SCr) and blood urea nitrogen (BUN) levels ind-gal mice (p <0.05). CGA also restored superoxide dismutase, catalase, and malondialdehyde levels and decreased glutathione content in the liver and kidney ind-gal mice (p <0.05). Improvements in liver and kidney were also noted in histopathological studies. CGA reduced tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) protein levels in the liver and kidney ind-gal mice (p <0.05). Discussion and conclusionThese findings suggest that CGA attenuatesd-gal-induced chronic liver and kidney injury and that this protection may be due to its antioxidative and anti-inflammatory activities. [ABSTRACT FROM AUTHOR]