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The correlation between miR-146a C/G polymorphism and UHRF1 gene expression level in gastric tumor.
- Source :
- Journal of Digestive Diseases; Mar2016, Vol. 17 Issue 3, p169-174, 6p
- Publication Year :
- 2016
-
Abstract
- Objective To investigate the association between the polymorphism of miR-146a and The ubiquitin-like with PHD and ring-finger domains 1 ( UHRF1) expression in patients with gastric cancer. Methods MiR-146a rs2910164 was genotyped in 130 patients with gastric cancer and 130 cancer-free individuals using polymerase chain reaction (PCR)-restriction fragment length polymorphism. UHRF1 expression was analyzed in 22 gastric cancer tissues and their adjacent normal tissues using quantitative real-time PCR. Results No significant differences in genotype distributions of miR-146a rs2910164 were found between cases and controls, but we observed that grade II tumors were more frequently detected in patients with CG/CC genotype compared to those with CC genotype. UHRF1 expressions in cancerous tissues were significantly higher than in noncancerous tissues (1.89-fold). Patients with CC genotype showed a significant increase in UHRF1 expression in comparison to the carriers of GG/CG genotype. A higher UHRF1 expression was associated with cancer stage IV and grade III ( P<0.05). Conclusion The overexpression of UHRF1 was correlated with the stage and grade of gastric cancer and is associated with the genotype distribution of rs2910164. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17512972
- Volume :
- 17
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of Digestive Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 114883644
- Full Text :
- https://doi.org/10.1111/1751-2980.12329