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CLONING AND EXPRESSION OF CAMEL (CAMELUS DROMEDARIUS) TH2 CYTOKINE GENE (IL-10) IN ECHERICHIA COLI DH5α CELLS.
- Source :
- Veterinary Practitioner; Jun2015, Vol. 16 Issue 1, p49-51, 3p
- Publication Year :
- 2015
-
Abstract
- In the present study peripheral blood mononuclear cells were separated from camel (Camelus dromedaries) blood, cultured and stimulated with Concavalin-A to maximize the expression of genes. Total RNA was separated and cDNA was synthesized using reverse transcriptase. The primers were designed for specific amplification of interleukin-10 gene of C. dromedaries using base sequence of C. bactrianus. The IL-10 gene was successfully amplified from cDNA and was identified on the basis of its size homology with that of C. bactrianus in agarose gel electrophoresis i.e. 600 bp. The IL-10 gene of C. dromedarius was cloned in pGEM-T easy vector and transformed in Escherichia coli DH5a strains. The cells containing recombinant plasmid could be identified on the basis of blue/white colony selection on LB agar containing X-Gal and ampicillin. Using the ideal camel model, further study of IL-10 characterization and function may provide insight on the understanding of the cell mediated immune system in camel. [ABSTRACT FROM AUTHOR]
- Subjects :
- CAMELS
INTERLEUKIN-10
ESCHERICHIA coli
AMPICILLIN
PLASMIDS
Subjects
Details
- Language :
- English
- ISSN :
- 09724036
- Volume :
- 16
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Veterinary Practitioner
- Publication Type :
- Academic Journal
- Accession number :
- 115960040