CLONING AND EXPRESSION OF CAMEL (CAMELUS DROMEDARIUS) TH2 CYTOKINE GENE (IL-10) IN ECHERICHIA COLI DH5α CELLS.

In the present study peripheral blood mononuclear cells were separated from camel (Camelus dromedaries) blood, cultured and stimulated with Concavalin-A to maximize the expression of genes. Total RNA was separated and cDNA was synthesized using reverse transcriptase. The primers were designed for specific amplification of interleukin-10 gene of C. dromedaries using base sequence of C. bactrianus. The IL-10 gene was successfully amplified from cDNA and was identified on the basis of its size homology with that of C. bactrianus in agarose gel electrophoresis i.e. 600 bp. The IL-10 gene of C. dromedarius was cloned in pGEM-T easy vector and transformed in Escherichia coli DH5a strains. The cells containing recombinant plasmid could be identified on the basis of blue/white colony selection on LB agar containing X-Gal and ampicillin. Using the ideal camel model, further study of IL-10 characterization and function may provide insight on the understanding of the cell mediated immune system in camel. [ABSTRACT FROM AUTHOR]