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T-helper-associated cytokines expression by peripheral blood mononuclear cells in patients with polypoidal choroidal vasculopathy and age-related macular degeneration.
- Source :
- BMC Ophthalmology; 6/7/2016, Vol. 16, p1-7, 7p
- Publication Year :
- 2016
-
Abstract
- <bold>Background: </bold>Immune responses play a key role in the pathogenesis and progression of polypoidal choroidal vasculopath (PCV) and age-related macular degeneration (AMD). In this study, we determined the Th cell-associated immune responses by measuring the cytokine expression of peripheral blood mononuclear cells (PBMC) in both PCV and neovascular AMD (nAMD) patients.<bold>Methods: </bold>Twenty-seven nAMD patients, 33 PCV patients and a gender- and age-matched group of 18 healthy individuals were involved in this study. The Th-cell cytokine profiles including levels of interferon-gamma (INF-γ), interleukin (IL)-17A and IL-4 in cultures of PBMCs were determined by enzyme-linked immunosorbent assay (ELISA).<bold>Results: </bold>IFN-γ,IL-17A and IL-4 production was significantly increased after stimulation with PHA. The levels of IFN-γ and IL-4 in PHA-stimulated cultures were higher in PCV and nAMD patients than that in healthy controls (P = 0.038,P = 0.014), while no difference was found between PCV and nAMD (all P > 0.05). No significant difference in IL-17A level in PHA-stimulated cultures was found among PCV, nAMD and control groups (P > 0.05).<bold>Conclusions: </bold>These findings suggest that circulating IFN-γ and IL-4 producing Th1 and Th2 cells may involve in the pathogenesis of PCV and nAMD. PCV may have the similar immune responses with nAMD. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14712415
- Volume :
- 16
- Database :
- Complementary Index
- Journal :
- BMC Ophthalmology
- Publication Type :
- Academic Journal
- Accession number :
- 116066668
- Full Text :
- https://doi.org/10.1186/s12886-016-0251-z