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CD1c-Expression by Monocytes – Implications for the Use of Commercial CD1c+ Dendritic Cell Isolation Kits.
- Source :
- PLoS ONE; 6/16/2016, Vol. 11 Issue 6, p1-15, 15p
- Publication Year :
- 2016
-
Abstract
- Conventional dendritic cells (cDCs) comprise a heterogeneous population of cells that are important regulators of immunity and homeostasis. CD1c<superscript>+</superscript> cDCs are present in human blood and tissues, and found to efficiently activate naïve CD4<superscript>+</superscript> T cells. While CD1c is thought to specifically identify this subset of human cDCs, we show here that also classical and intermediate monocytes express CD1c. Accordingly, the commercial CD1c (BDCA-1)<superscript>+</superscript> Dendritic Cell Isolation Kit isolates two distinct cell populations from blood: CD1c<superscript>+</superscript>CD14<superscript>−</superscript> cDCs and CD1c<superscript>+</superscript>CD14<superscript>+</superscript> monocytes. CD1c<superscript>+</superscript> cDCs and CD1c<superscript>+</superscript> monocytes exhibited strikingly different properties, including their differential regulation of surface marker expression, their levels of cytokine production, and their ability to stimulate naïve CD4<superscript>+</superscript> T cells. These results demonstrate that a commercial CD1c (BDCA-1)<superscript>+</superscript> Dendritic Cell Isolation Kit isolates two functionally different cell populations, which has important implications for the interpretation of previously generated data using this kit to characterize CD1c<superscript>+</superscript> cDCs. [ABSTRACT FROM AUTHOR]
- Subjects :
- MONOCYTES
DENDRITIC cells
HOMEOSTASIS
T cells
CELL surface antigens
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 116190419
- Full Text :
- https://doi.org/10.1371/journal.pone.0157387