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Multimodal In Vivo Imaging of Tumorigenesis and Response to Chemotherapy in a Transgenic Mouse Model of Mammary Cancer.
- Source :
- Molecular Imaging & Biology; Aug2016, Vol. 18 Issue 4, p617-626, 10p
- Publication Year :
- 2016
-
Abstract
- <bold>Purpose: </bold>Transgenic mice expressing the polyoma middle T oncoprotein (PyMT) in the mammary epithelium were explored by multimodal imaging to monitor longitudinally spontaneous tumor growth and response to chemotherapy.<bold>Procedures: </bold>Positron emission tomography (PET) with 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) and 3'-deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT), single photon emission tomography (SPECT) with [(99m)Tc]TcO4 ([(99m)Tc]TEC), X-ray computed tomography, and fluorescent confocal endomicroscopy (FCE) images were acquired during tumor progression in female PyMT mice. Imaging with [(18)F]FDG and [(99m)Tc]TEC was also performed in untreated, doxorubicin-treated, and docetaxel-treated PyMT mice. Total tumor volumes were quantified. Tumors were collected and macroscopic and histological examinations were performed.<bold>Results: </bold>All PyMT mice developed multifocal tumors of the mammary epithelium that became palpable at 8 weeks of age (W8). Computed tomography (CT) detected tumors at W14, while a clear tumoral uptake of [(99m)Tc]TEC and [(18)F]FDG was present as early as W6 and W8, respectively. No contrast between mammary tumors and surrounding tissue was observed at any stage with [(18)F]FLT. FCE detected an angiogenic switch at W10. Lung metastases were not clearly evidenced by imaging. Doxorubicin and docetaxel treatments delayed tumor growth, as shown by [(18)F]FDG and [(99m)Tc]TEC, but tumor growth resumed upon treatment discontinuation. Tumor growth fitted an exponential model with time constant rates of 0.315, 0.145, and 0.212 week(-1) in untreated, doxorubicin, and docetaxel groups, respectively.<bold>Conclusions: </bold>Molecular imaging of mammary tumors in PyMT is precocious, precise, and predictive. [(18)F]FDG-PET and [(99m)Tc]TEC SPECT monitor tumor response to chemotherapy. [ABSTRACT FROM AUTHOR]
- Subjects :
- NEOPLASTIC cell transformation
LABORATORY mice
CANCER chemotherapy
DRUG therapy
ANTINEOPLASTIC agents
CARCINOGENESIS
ANIMAL experimentation
BIOLOGICAL models
CELL physiology
COMPUTED tomography
DEOXY sugars
DIAGNOSTIC imaging
LIGHT
MICE
RADIOPHARMACEUTICALS
TECHNETIUM
TIME
TUMOR markers
SINGLE-photon emission computed tomography
DISEASE progression
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 15361632
- Volume :
- 18
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Molecular Imaging & Biology
- Publication Type :
- Academic Journal
- Accession number :
- 116510049
- Full Text :
- https://doi.org/10.1007/s11307-015-0916-7