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An engineered non-oxidative glycolysis pathway for acetone production in Escherichia coli.
- Source :
- Biotechnology Letters; Aug2016, Vol. 38 Issue 8, p1359-1365, 7p
- Publication Year :
- 2016
-
Abstract
- Objectives: To find new metabolic engineering strategies to improve the yield of acetone in Escherichia coli. Results: Results of flux balance analysis from a modified Escherichia coli genome-scale metabolic network suggested that the introduction of a non-oxidative glycolysis (NOG) pathway would improve the theoretical acetone yield from 1 to 1.5 mol acetone/mol glucose. By inserting the fxpk gene encoding phosphoketolase from Bifidobacterium adolescentis into the genome, we constructed a NOG pathway in E.coli. The resulting strain produced 47 mM acetone from glucose under aerobic conditions in shake-flasks. The yield of acetone was improved from 0.38 to 0.47 mol acetone/mol glucose which is a significant over the parent strain. Conclusions: Guided by computational analysis of metabolic networks, we introduced a NOG pathway into E. coli and increased the yield of acetone, which demonstrates the importance of modeling analysis for the novel metabolic engineering strategies. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01415492
- Volume :
- 38
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Biotechnology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 116775359
- Full Text :
- https://doi.org/10.1007/s10529-016-2115-2