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Immobilization of the Gas Signaling Molecule H2S by Radioisotopes: Detection, Quantification, and In Vivo Imaging.
- Source :
- Angewandte Chemie International Edition; 8/1/2016, Vol. 55 Issue 32, p9365-9370, 6p
- Publication Year :
- 2016
-
Abstract
- Hydrogen sulfide (H<subscript>2</subscript>S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H<subscript>2</subscript>S in live animals is very challenging. Herein, we report the first radioisotope-based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H<subscript>2</subscript>S. Macrocyclic <superscript>64</superscript>Cu complexes that instantly reacted with gaseous H<subscript>2</subscript>S to form insoluble <superscript>64</superscript>CuS in a highly sensitive and selective manner were prepared. The H<subscript>2</subscript>S concentration in biological samples was measured by a thin-layer radiochromatography method. When <superscript>64</superscript>Cu-cyclen was injected into mice, an elevated H<subscript>2</subscript>S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H<subscript>2</subscript>S levels in a millimeter-sized infarcted lesion of the rat heart. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 55
- Issue :
- 32
- Database :
- Complementary Index
- Journal :
- Angewandte Chemie International Edition
- Publication Type :
- Academic Journal
- Accession number :
- 117066033
- Full Text :
- https://doi.org/10.1002/anie.201603813