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Antibacterial activity of oxyresveratrol against methicillin-resistant Staphylococcus aureus and its mechanism.

Authors :
DAE-KI JOUNG
SU-HYUN MUN
SUNG-HOON CHOI
OK-HWA KANG
SUNG-BAE KIM
YOUNG-SEOB LEE
TIAN ZHOU
RYONG KONG
JANG-GI CHOI
DONG-WON SHIN
YOUN-CHUL KIM
DONG-SUNG LEE
DONG-YEUL KWON
Source :
Experimental & Therapeutic Medicine; Sep2016, Vol. 12 Issue 3, p1579-1584, 6p
Publication Year :
2016

Abstract

Oxyresveratrol (ORV) is a naturally occurring compound found in mulberries that exhibits a wide spectrum of biological activities. However, the underlying mechanism of the action of ORV against the methicillin-resistant S. aureus (MRSA) pathogen has not yet been reported. MRSA is multidrug-resistant, causing skin and other types of infections. The aim of the present study was to examine the antimicrobial activity of ORV and the underlying mechanism of its action on MRSA. The antibacterial activity of ORV was evaluated using a minimum inhibitory concentration (MIC) assay, and the mechanism of its antibacterial action on S. aureus was investigated using a combination of ORV with detergent, ATPase inhibitors and peptidoglycan (PGN). In addition, the survival characteristics and changes in MRSA morphology were monitored using transmission electron microscopy (TEM). The MIC value of ORV against all S. aureus strains was found to be 125 µg/ml. The optical density at 600 nm of each suspension treated using a combination of ORV with Triton X-100, N,N'-dicyclohexylcarbodiimide or sodium azide was reduced by 68.9-89.8% compared with the value upon treatment with ORV alone. In the ORV and PGN combination assay, direct binding of ORV with PGN from S. aureus was evident. Furthermore, TEM examination of MRSA treated with ORV showed alterations in septa formation. In conclusion, these results showed that ORV has a strong antibacterial effect against S. aureus, mainly by increasing membrane permeability and inhibiting ATPase when combined with other drugs. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17920981
Volume :
12
Issue :
3
Database :
Complementary Index
Journal :
Experimental & Therapeutic Medicine
Publication Type :
Academic Journal
Accession number :
117223254
Full Text :
https://doi.org/10.3892/etm.2016.3486