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Brief communication Rapid detection of all HLA-B*27 alleles (B*2701–B*2725) by group-specific polymerase chain reaction.
- Source :
- Tissue Antigens; Jan2004, Vol. 63 Issue 1, p88-92, 5p
- Publication Year :
- 2004
-
Abstract
- Human leucocyte antigen-B*27 is strongly associated with a number of rheumatic diseases, including ankylosing spondylitis and reactive arthritis. Targeted detection of the B*27 group by molecular methods is hampered by the extreme heterogeneity of the serological B*27 group. Here, we describe a simple, rapid sequence-specific primer-based method for detection of all 28 B*27 alleles defined to date. The method involves an initial screening with two sequence-specific polymerase chain reactions (PCRs), which has to be followed by two additional PCR amplifications in samples carrying a few rare subtypes of B*27, B*4202 or B*7301. The described protocol should be useful for laboratories involved in diagnostics and research of rheumatoid diseases. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00012815
- Volume :
- 63
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Tissue Antigens
- Publication Type :
- Academic Journal
- Accession number :
- 11784431
- Full Text :
- https://doi.org/10.1111/j.1399-0039.2004.00158.x