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easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy.

Authors :
Kwakwa, Kwasi
Savell, Alexander
Davies, Timothy
Munro, Ian
Parrinello, Simona
Purbhoo, Marco A.
Dunsby, Chris
Neil, Mark A.A.
French, Paul M.W.
Source :
Journal of Biophotonics; Sep2016, Vol. 9 Issue 9, p948-957, 10p
Publication Year :
2016

Abstract

TIRF and STORM microscopy are super-resolving fluorescence imaging modalities for which current implementations on standard microscopes can present significant complexity and cost. We present a straightforward and low-cost approach to implement STORM and TIRF taking advantage of multimode optical fibres and multimode diode lasers to provide the required excitation light. Combined with open source software and relatively simple protocols to prepare samples for STORM, including the use of Vectashield for non-TIRF imaging, this approach enables TIRF and STORM imaging of cells labelled with appropriate dyes or expressing suitable fluorescent proteins to become widely accessible at low cost. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1864063X
Volume :
9
Issue :
9
Database :
Complementary Index
Journal :
Journal of Biophotonics
Publication Type :
Academic Journal
Accession number :
117898647
Full Text :
https://doi.org/10.1002/jbio.201500324