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Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
- Source :
- PLoS ONE; 9/9/2016, Vol. 11 Issue 9, p1-16, 16p
- Publication Year :
- 2016
-
Abstract
- As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8<superscript>+</superscript> T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8<superscript>+</superscript> T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8<superscript>+</superscript> T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8<superscript>+</superscript> T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8<superscript>+</superscript> T cell responses linked to specific MHC class I alleles. [ABSTRACT FROM AUTHOR]
- Subjects :
- THEILERIA
ANTIGEN analysis
T cells
CD8 antigen
MAJOR histocompatibility complex
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 117993948
- Full Text :
- https://doi.org/10.1371/journal.pone.0162571