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Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest.
- Source :
- Chinese Journal of Integrative Medicine; Sep2016, Vol. 22 Issue 9, p666-673, 8p
- Publication Year :
- 2016
-
Abstract
- Objective: To investigate whether Shen-Fu Injection (参附注射液, SFI) reduces post-resuscitation immune dysfunction in a porcine model of cardiac arrest by modulating apoptosis of regulatory T lymphocytes (Treg) in the spleen. Methods: After 8-min untreated ventricular fibrillation and 2-min basic life support, 24 pigs were divided into 3 groups with a random number table, i.e. SFI group, epinephrine (EP) group, and saline (SA) group (8 in each group), which received central venous injection of SFI (1.0 mL/kg), EP (0.02 mg/kg) and SA, respectively. The same procedure without CA initiation was achieved in the sham-operated (sham) group ( n=6). After successful return of spontaneous circulation (ROSC), apoptosis rate of splenic Treg was detected by flow cytometry; and the mRNA expression of forkhead/winged helix transcription factor (Foxp3) of splenic Treg was detected by real time-polymerase chain reaction; and the levels of interleukin-4 (IL-4) and interferon-γ (IFN-γ) in porcine splenic Treg were detected by using enzyme-linked immunosorbent assay (ELISA). Results: Compared with the sham group, the apoptosis rate of Treg was significantly decreased, and the levels of Foxp3 mRNA expression, IFN-γ, IL-4 and IFN-γ/IL-4 were increased in the SA group ( P<0.05 or P<0.01). Compared with the EP and SA groups, SFI treatment increased the apoptosis rate of Treg and reduced the levels of Foxp3 mRNA expression, IFN-γ and IFN-γ/IL-4 ( P<0.05). Conclusions: SFI has signifificant effects in attenuating post-resuscitation immune dysfunction by modulating apoptosis of Treg in the spleen. [ABSTRACT FROM AUTHOR]
- Subjects :
- CARDIAC arrest
SPLEEN
OXYGEN metabolism
ANALYSIS of variance
ANIMAL experimentation
APOPTOSIS
CELL culture
CARDIOPULMONARY resuscitation
CYTOKINES
ENZYME-linked immunosorbent assay
FLOW cytometry
HEMODYNAMICS
IMMUNE system
INJECTIONS
INTERLEUKINS
LIFE support systems in critical care
CHINESE medicine
POLYMERASE chain reaction
RESUSCITATION
RNA
SURVIVAL
SWINE
T cells
T-test (Statistics)
VENTRICULAR fibrillation
DATA analysis
CONTROL groups
DATA analysis software
CD4 lymphocyte count
ANATOMY
DIAGNOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 16720415
- Volume :
- 22
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Chinese Journal of Integrative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 118007815
- Full Text :
- https://doi.org/10.1007/s11655-016-2505-2