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Assay Establishment and Validation of a High-Throughput Screening Platform for Three-Dimensional Patient-Derived Colon Cancer Organoid Cultures.
- Source :
- Journal of Biomolecular Screening; Oct2016, Vol. 21 Issue 9, p931-941, 11p
- Publication Year :
- 2016
-
Abstract
- The application of patient-derived three-dimensional culture systems as disease-specific drug sensitivity models has enormous potential to connect compound screening and clinical trials. However, the implementation of complex cell-based assay systems in drug discovery requires reliable and robust screening platforms. Here we describe the establishment of an automated platform in 384-well format for three-dimensional organoid cultures derived from colon cancer patients. Single cells were embedded in an extracellular matrix by an automated workflow and subsequently self-organized into organoid structures within 4 days of culture before being exposed to compound treatment. We performed validation of assay robustness and reproducibility via plate uniformity and replicate-experiment studies. After assay optimization, the patient-derived organoid platform passed all relevant validation criteria. In addition, we introduced a streamlined plate uniformity study to evaluate patient-derived colon cancer samples from different donors. Our results demonstrate the feasibility of using patient-derived tumor samples for high-throughput assays and their integration as disease-specific models in drug discovery. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10870571
- Volume :
- 21
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Journal of Biomolecular Screening
- Publication Type :
- Academic Journal
- Accession number :
- 118237453
- Full Text :
- https://doi.org/10.1177/1087057116650965