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Baicalein Promotes Angiogenesis and Odontoblastic Differentiation via the BMP and Wnt Pathways in Human Dental Pulp Cells.
- Source :
- American Journal of Chinese Medicine; 2016, Vol. 44 Issue 7, p1457-1472, 16p, 3 Diagrams, 1 Chart, 4 Graphs
- Publication Year :
- 2016
-
Abstract
- Baicalein is an active flavonoid extracted from the root of Scutellaria baicalensis that has anticancer and anti-inflammatory properties; its effects on osteoblastic and angiogenic potential are controversial. The aim of this study was to investigate the effects of baicalein on odontoblastic differentiation and angiogenesis and the underlying mechanism in human dental pulp cells (HDPCs). Baicalein (1-10M) had no cytotoxic effects and promoted alkaline phosphatase (ALP) activity, mineralization assayed by Alizarin Red-S staining, and the mRNA expression of marker genes, in a concentration-dependent manner. In addition, baicalein upregulated angiogenic factors and increased in vitro capillary-like tube formation. Moreover, baicalein upregulated bone morphogenetic protein (BMP)-2 mRNA and phosphorylation of Smad 1/5/8 and Wnt ligand mRNA, glycogen synthase kinase-3, and nuclear -catenin. The odontogenic and angiogenic effects of baicalein were abolished by the BMP antagonist noggin and the Wnt/-catenin receptor antagonist DKK-1. These results demonstrate that baicalein promoted odontoblastic differentiation and angiogenesis of HDPCs by activating the BMP and Wnt/-catenin signal pathways. Our findings suggest that baicalein may contribute to dental pulp repair and regenerative endodontics. [ABSTRACT FROM AUTHOR]
- Subjects :
- ENZYME metabolism
ALKALINE phosphatase
ALTERNATIVE medicine
BIOLOGICAL models
BONE morphogenetic proteins
CELL differentiation
CELLULAR signal transduction
DENTAL pulp
DOSE-effect relationship in pharmacology
FLAVONOIDS
MEDICINAL plants
NEOVASCULARIZATION
PHOSPHORYLATION
POLYMERASE chain reaction
PROBABILITY theory
RESEARCH funding
PLANT roots
STAINS & staining (Microscopy)
STATISTICS
WESTERN immunoblotting
PLANT extracts
DATA analysis
REVERSE transcriptase polymerase chain reaction
REMINERALIZATION (Teeth)
DESCRIPTIVE statistics
IN vitro studies
ONE-way analysis of variance
Subjects
Details
- Language :
- English
- ISSN :
- 0192415X
- Volume :
- 44
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- American Journal of Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 119232264
- Full Text :
- https://doi.org/10.1142/S0192415X16500816