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Autophagy Protects from Raddeanin A-Induced Apoptosis in SGC-7901 Human Gastric Cancer Cells.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 11/15/2016, Vol. 2016, p1-8, 8p, 1 Diagram, 2 Graphs
- Publication Year :
- 2016
-
Abstract
- Raddeanin A (RA) is an extractive from Anemone raddeana Regel, a traditional Chinese medicine. The aim of this study is to assess the efficacy of RA against human gastric cancer (GC) cells (SGC-7901) and explore its mechanism. MTT assay showed that RA inhibition of proliferation of SGC-7901 cells increased in a dose-dependent manner. Flow cytometry analysis and Hoechst 33258 staining showed that RA induced apoptosis on SGC-7901 cells. Meanwhile, it induced autophagy. Western blotting analysis showed that the RA induces apoptosis and autophagy by activating p38 MAPK pathway and inhibiting mTOR pathway. Further studies showed that autophagy inhibition could protect from RA-induced apoptosis in SGC-7901 cells. In conclusion, RA can induce SGC-7901 cell apoptosis and autophagy by activating p38 MAPK pathway. And autophagy can protect SGC-7901 cells from apoptosis induced by RA. [ABSTRACT FROM AUTHOR]
- Subjects :
- GASTROINTESTINAL tumors
AUTOPHAGY
ALTERNATIVE medicine
ANTINEOPLASTIC agents
APOPTOSIS
BIOLOGICAL assay
BIOLOGICAL models
DOSE-effect relationship in pharmacology
FLOW cytometry
MEDICINAL plants
PROTEIN kinases
STAINS & staining (Microscopy)
WESTERN immunoblotting
PLANT extracts
IN vitro studies
PHARMACODYNAMICS
PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Volume :
- 2016
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 119474870
- Full Text :
- https://doi.org/10.1155/2016/9406758