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A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability.
- Source :
- PLoS ONE; 12/15/2016, Vol. 11 Issue 12, p1-8, 8p
- Publication Year :
- 2016
-
Abstract
- This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS) components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB) that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size. [ABSTRACT FROM AUTHOR]
- Subjects :
- CANCER cells
FLUOROPHORES
PROTOTYPES
FLUORESCENCE microscopy
REAL-time computing
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 120227798
- Full Text :
- https://doi.org/10.1371/journal.pone.0167863