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Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass.
- Source :
- Obesity (19307381); Jan2017, Vol. 25 Issue 1, p102-110, 9p
- Publication Year :
- 2017
-
Abstract
- <bold>Objective: </bold>Exosomes from obese adipose contain dysregulated microRNAs linked to insulin signaling, as compared with lean controls, providing a direct connection between adiposity and insulin resistance. This study tested the hypotheses that gastric bypass surgery and its subsequent weight loss would normalize adipocyte-derived exosomal microRNAs associated with insulin signaling and the associated metabolome related to glucose homeostasis.<bold>Methods: </bold>African American female subjects with obesity (N = 6; age: 38.5 ± 6.8 years; BMI: 51.2 ± 8.8 kg/m2 ) were tested before and 1 year after surgery. Insulin resistance (HOMA), serum metabolomics, and global microRNA profiles of circulating adipocyte-derived exosomes were evaluated via ANCOVA and correlational analyses.<bold>Results: </bold>One year postsurgery, patients showed decreased BMI (-18.6 ± 5.1 kg/m2 ; P < 0.001), ameliorated insulin resistance (HOMA: 1.94 ± 0.6 presurgery, 0.49 ± 0.1 postsurgery; P < 0.001), and altered metabolites including branched chain amino acids (BCAA). Biological pathway analysis of predicted mRNA targets of 168 surgery-responsive microRNAs (P < 0.05) identified the insulin signaling pathway (P = 1.27E-10; 52/138 elements), among others, in the data set. The insulin signaling pathway was also a target of 10 microRNAs correlated to changes in HOMA (P < 0.05; r > 0.4), and 48 microRNAs correlated to changes in BCAA levels.<bold>Conclusions: </bold>These data indicate that circulating adipocyte-derived exosomes are modified following gastric bypass surgery and correlate to improved postsurgery insulin resistance. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19307381
- Volume :
- 25
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Obesity (19307381)
- Publication Type :
- Academic Journal
- Accession number :
- 120386211
- Full Text :
- https://doi.org/10.1002/oby.21709