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Three-dimensional imaging of the intracellular assembly of a functional viral RNA replicase complex.
- Source :
- Journal of Cell Science; 1/1/2017, Vol. 130 Issue 1, p260-268, 9p
- Publication Year :
- 2017
-
Abstract
- Positive-strand RNA viruses, which can be devastating pathogens in humans, animals and plants, replicate their genomes on intracellular membranes. Here, we describe the three-dimensional ultrastructural organization of a tombusvirus replicase in yeast, a valuable model for exploring virus-host interactions. We visualized the intracellular distribution of a viral replicase protein using metal-tagging transmission electron microscopy, a highly sensitive nanotechnology whose full potential remains to be developed. These three-dimensional images show how viral replicase molecules are organized when they are incorporated into the active domains of the intracellular replication compartment. Our approach provides a means to study protein activation mechanisms in cells and to identify targets for new antiviral compounds. [ABSTRACT FROM AUTHOR]
- Subjects :
- VIRAL replication
RNA replicase
THREE-dimensional imaging in biology
Subjects
Details
- Language :
- English
- ISSN :
- 00219533
- Volume :
- 130
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Cell Science
- Publication Type :
- Academic Journal
- Accession number :
- 120606540
- Full Text :
- https://doi.org/10.1242/jcs.181586