Evaluation of robot-assisted laparoscopic versus open cystectomy and effect of carbon dioxide-pneumoperitoneum on histopathological findings in ureteroenteric anastomoses: results from an experimental randomized porcine study.

Objective: The aim of this study was to investigate the histopathology of ureteroenteric anastomoses (UEAs) after radical cystectomy (RC) with an ileal conduit in a porcine model using different surgical techniques. The study also evaluated the potential anti-inflammatory effect of preoperative carbon dioxide-pneumoperitoneum (CO₂P). Materials and methods: Forty female Danish Landrace pigs were randomized to four groups: three intervention groups [open surgery ± prior CO₂P and robot-assisted laparoscopic cystectomy (RALC)], all subjected to RC and an ileal conduit, and one control group. After euthanization, UEAs were harvested and histopathologically evaluated. Results: Five pigs were excluded from the study owing to postoperative complications. Operating room time and clamping of the right and left ureters were significantly longer in the robotic than in the open groups (p <.01). Inflammatory infiltration grade of the left ureter was significantly higher in RALC (p =.032). No statistically significant difference was observed between the open groups receiving prior CO₂P or not. Conclusions: Results showed a statistically significant higher inflammatory infiltration grade of the left ureter in RALC, emphasizing the importance of using meticulous techniques when mobilizing the left ureter using the robot. It was not possible to conclude with certainty whether CO₂P could constitute an anti-inflammatory agent for local inflammation. [ABSTRACT FROM AUTHOR]