Back to Search
Start Over
Andrographolide induces apoptotic and non-apoptotic death and enhances tumor necrosis factor-related apoptosis-inducing ligand mediated apoptosis in gastric cancer cells.
- Source :
- Oncology Letters; May2017, Vol. 13 Issue 5, p3837-3844, 8p
- Publication Year :
- 2017
-
Abstract
- Andrographolide, a natural compound isolated from Andrographis paniculata, has been reported to possess antitumor activity. In the present study, the effect of andrographolide in human gastric cancer (GC) cells was investigated. Andrographolide induced cell death with apoptotic and non-apoptotic features. At a low concentration, andrographolide potentiated apoptosis and reduction of clonogenicity triggered by recombinant human tumor necrosis factor-related apoptosis-inducing ligand (rhTRAIL). Exposure of GC cells to andrographolide altered the expression level of several growth-inhibiting and apoptosis-regulating proteins, including death receptors. It was demonstrated that activity of the TRAIL-R2 (DR5) pathway was critical in the development of andrographolide-mediated rhTRAIL sensitization, since its inhibition significantly reduced the extent of apoptosis induced by the combination of rhTRAIL and andrographolide. In addition, andrographolide increased reactive oxygen species (ROS) generation in a dose-dependent manner. N-acetyl cysteine prevented andrographolide-mediated DR5 induction and the apoptotic effect induced by the combination of rhTRAIL and andrographolide. Collectively, the present study demonstrated that andrographolide enhances TRAIL-induced apoptosis through induction of DR5 expression. This effect appears to involve ROS generation in GCs. [ABSTRACT FROM AUTHOR]
- Subjects :
- APOPTOSIS
STOMACH cancer
TUMOR necrosis factors
CANCER cells
CELL death
Subjects
Details
- Language :
- English
- ISSN :
- 17921074
- Volume :
- 13
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Oncology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 122571248
- Full Text :
- https://doi.org/10.3892/ol.2017.5923