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A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione.
- Source :
- Angewandte Chemie; 5/15/2017, Vol. 129 Issue 21, p5906-5910, 5p
- Publication Year :
- 2017
-
Abstract
- The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG- 1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant ( K<subscript>d</subscript>) of 2.59 m m and a fast response time ( t<subscript>1/2</subscript>=5.82 s). We also demonstrate that QG- 1 detection of GSH is feasible in a model protein system. QG- 1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 m m). [ABSTRACT FROM AUTHOR]
- Subjects :
- FLUORESCENCE
GLUTATHIONE
CELL-mediated cytotoxicity
BIOCHEMISTRY
ANTIOXIDANTS
Subjects
Details
- Language :
- English
- ISSN :
- 00448249
- Volume :
- 129
- Issue :
- 21
- Database :
- Complementary Index
- Journal :
- Angewandte Chemie
- Publication Type :
- Academic Journal
- Accession number :
- 122918662
- Full Text :
- https://doi.org/10.1002/ange.201702114