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Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in Brassica napus L.

Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in Brassica napus L.

Authors :
Heng, Shuangping
Chen, Fengyi
Wei, Chao
Hu, Kaining
Yang, Zonghui
Wen, Jing
Yi, Bin
Ma, Chaozhi
Tu, Jinxing
Si, Ping
Fu, Tingdong
Shen, Jinxiong
Source :
Plant Cell Reports; Jun2017, Vol. 36 Issue 6, p901-909, 9p
Publication Year :
2017

Abstract

Key message: Different mitotype-specific markers were developed to distinguish different cytoplasms in Brassica napus L. Abstract: Mitotype-specific markers have been developed to distinguish different mitotypes in plant. And use of molecular markers to identify different mitotypes in Brassica napus would enhance breeding efficiency. Here, we comparatively analyzed six sequenced mitochondrial genomes in Brassica napus and identified collinear block sequences and mitotype-specific sequences (MSSs) of these mitochondrial genomes. The collinear block sequences between mitochondrial genomes of nap, cam, and pol cytoplasmic male sterility (CMS) lines were higher than those of other lines. After comparative analysis of the six sequenced mitochondrial genomes ( cam, nap, ole, pol CMS, ogu CMS, and hau CMS), 90 MSSs with sizes ranging from 101 to 9981 bp and a total length of 103,756 bp (accounting for 6.77% of the mitochondrial genome sequences) were identified. Additionally, 12 mitotype-specific markers were developed based on the mitochondrial genome-specific sequences in order to distinguish among these different mitotypes. Cytoplasms of 570 different inbred lines collected across scientific research institutes in China were identified using the MSS markers developed in our study. In addition to confirming the accuracy of the cytoplasmic identification, we also identified mitotypes that have not been reported in Brassica napus. Our study may provide guidance for the classification of different mitotypes in B. napus breeding. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07217714
Volume :
36
Issue :
6
Database :
Complementary Index
Journal :
Plant Cell Reports
Publication Type :
Academic Journal
Accession number :
123325269
Full Text :
https://doi.org/10.1007/s00299-017-2121-4