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A gene cluster at an unusual chromosomal location responsible for the novel O-antigen synthesis in Escherichia coli O62 by the ABC transporter-dependent pathway.
- Source :
- Glycobiology; Jul2017, Vol. 27 Issue 7, p669-676, 8p
- Publication Year :
- 2017
-
Abstract
- The O-antigen is a part of the outer membrane of Gram-negative bacteria and is related to bacterial virulence. It is one of the most variable cell constituents, and its structural diversity is almost entirely due to genetic variation of the O-antigen gene cluster. In this study, the O-antigen structure of Escherichia coli O62 was elucidated by chemical analysis and nuclear magnetic resonance spectroscopy, but showing not consistent with the O-antigen gene cluster between conserved genes galF and gnd reported earlier. The complete genome of E. coli O62 was then sequenced and analyzed, and another O-antigen gene cluster was found and characterized that correlated perfectly with the established O-antigen structure. A deletion and complementation experiment confirmed the functionality of the novel gene cluster and demonstrated that the O62-antigen is synthesized by the ABC transporter-dependent system. To our knowledge, this is the first report that the O-antigen gene cluster is positioned at a novel locus in E. coli. Comparative analysis indicated that E. coli O62 likely originated from E. coli O68 via an IS event resulting in the repression of the O68-antigen synthesis, followed by the acquisition of a novel O-antigen gene cluster from Enterobacter aerogenes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09596658
- Volume :
- 27
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Glycobiology
- Publication Type :
- Academic Journal
- Accession number :
- 123455474
- Full Text :
- https://doi.org/10.1093/glycob/cwx030