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Platinum nanoparticle-assembled nanoflake-like tin disulfide for enzyme-based amperometric sensing of glucose.
- Source :
- Microchimica Acta; Jul2017, Vol. 184 Issue 7, p2357-2363, 7p
- Publication Year :
- 2017
-
Abstract
- The authors have prepared a platinum nanoparticle-assembled nanoflake-like SnS nanocomposite of the type PtNP@SnS as a support for immobilization of glucose oxidase (GOx) to obtain an electrochemical glucose biosensor based on direct electron transfer of GOx. Scanning electron microscopy, transmission electron microscopy, X-ray diffraction, electrochemical impedance spectroscopy, static water contact angle measurements, and cyclic voltammetry were used to characterize the nanocomposite and the glucose biosensor. The nanocomposite has a large surface-to-volume ratio, excellent hydrophiliciy, and high electrochemical activity. This promotes the direct electron transfer between GOx and the surface of a glassy carbon electrode (GCE). The GOx immobilized on the nanocomposite displays good bioactivity, and the surface coverage of the modified electrode is 4.15 × 10 mol·cm. Under optimum conditions and at a working voltage of −0.4 V vs. SCE, the glucose biosensor exhibits a linear response in the 0.1 to 1.0 mM and 1.0 to 12 mM glucose concentration ranges, with a lower detection limit of 2.5 μM (at an S/N ratio of 3). The biosensor shows excellent selectivity and was successfully applied to the determination of glucose in human serum samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 184
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 123518879
- Full Text :
- https://doi.org/10.1007/s00604-017-2209-0