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Influence of substituents on DNA and protein binding of cyclometalated Ir(iii) complexes and anticancer activity.
- Source :
- Dalton Transactions: An International Journal of Inorganic Chemistry; 7/14/2017, Vol. 46 Issue 26, p8572-8585, 14p
- Publication Year :
- 2017
-
Abstract
- Synthesis of terpyridyl based ligands 3-([2,2′:6′,2′′-terpyridin]-4′-yl)-7-methoxy-2-(methylthio)-quinolone, (L1); 3-([2,2′:6′,2′′-terpyridin]-4′-yl)-6-methoxyquinolin-2(1H)-one, (L2); 3-([2,2′-:6′,2′′-terpyridin]-4′-yl)-6-methylquinolin-2(1H)-one (L3) and cyclometalated iridium(iii) complexes [[Ir(ppy)<subscript>2</subscript>L1]<superscript>+</superscript>PF<subscript>6</subscript><superscript>−</superscript> (1), [Ir(ppy)<subscript>2</subscript>L2]<superscript>+</superscript>PF<subscript>6</subscript><superscript>−</superscript> (2), [Ir(ppy)<subscript>2</subscript>L3]<superscript>+</superscript>PF<subscript>6</subscript><superscript>−</superscript> (3) (2-phenylpyridine = Hppy)] involving these ligands has been described. The ligands L1–L3 and complexes 1–3 have been thoroughly characterized by elemental analyses, spectral studies (IR, <superscript>1</superscript>H, <superscript>13</superscript>C NMR, UV/vis and fluorescence) ESI-MS, and the structure of 3 has been unambiguously authenticated by single crystal X-ray analyses. UV/vis, fluorescence and circular dichroism spectroscopic studies showed rather efficient binding of 1 with CT-DNA (calf thymus DNA) and BSA (bovine serum albumin) relative to 2 and 3. Molecular docking studies unveiled binding of 1–3 with minor groove of CT-DNA via van der Waal's forces and electrostatically with the hydrophobic moiety of HSA (human serum albumin). The ligands and complexes exhibited moderate cytotoxicity towards MDA-MB-231 (breast cancer cell line) and significant influence on HeLa (cervical cancer cell line) cells. Cytotoxicity, morphological changes, and apoptosis have been followed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) assay, Hoechst 33342/PI (PI = propidium iodide) staining, cell cycle analysis by FACS (fluorescence activated cell sorting), and ROS (reactive oxygen species) generation by DCFH-DA (dichlorodihydrofluorescein diacetate) dye. Confocal microscopy images revealed that the drug efficiently initiates apoptosis in the cell cytosol. The IC<subscript>50</subscript> values showed superior cytotoxicity of 1–3 against the HeLa cell line relative to cisplatin, and their ability to induce apoptosis is in the order 1 > 2 > 3. [ABSTRACT FROM AUTHOR]
- Subjects :
- IRIDIUM compounds
DNA-ligand interactions
ANTINEOPLASTIC agents
Subjects
Details
- Language :
- English
- ISSN :
- 14779226
- Volume :
- 46
- Issue :
- 26
- Database :
- Complementary Index
- Journal :
- Dalton Transactions: An International Journal of Inorganic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 123915521
- Full Text :
- https://doi.org/10.1039/c7dt01015j