Lack of association association between the presence of dupA and babA genes in Helicobacter pylori and gastroduodenal disorders.

Introduction and objective: Bacterial virulence factors are important in determining the outcome of H. pylori associated diseases. The initial stage of colonization is the binding of H. pylori to surface receptors on gastric epithelial cells known as bаbА. It caused colonization by another recently described H. pylori virulence factor, the duodenal ulcer promoter gene A, which is a specific marker for the development of duodenal ulcer and protection against gastric adenocarcinoma. Based on heterogeneity among H. pylori strains, this study was aimed at investigating the frequency of bаbА and dupA genes in clinical isolates of H. pylori and its correlation with different H. pylori associated diseases in Kermanshah, Iran. Methods: Stomach biopsy specimens were collected and inoculated into egg yolk and antibiotics supplemented Columbia agar. Presumptive colonies were confirmed biochemically and genetically, and the presence of bаbА and dupA genes was detected by PCR. Results: This study included a total of 96 isolates from patients with different gastroduodenal disorders. The prevalence of bаbА and dupA genes was observed to be 58.38 and 37.5%, respectively. Conclusion: Despite the fact that there was no statistically significant relationship between bаbА and dupA virulence factors and gastroduodenal diseases, a high frequency of the genes was recorded among the isolates in Iran. [ABSTRACT FROM AUTHOR]