Back to Search
Start Over
A rapid and high-throughput method for the determination of serum uric acid based on microarray technology and nanomaterial.
- Source :
- Luminescence: Journal of Biological & Chemical Luminescence; Aug2017, Vol. 32 Issue 5, p730-734, 5p
- Publication Year :
- 2017
-
Abstract
- Serum uric acid (SUA) is a new therapeutic target for non-alcoholic fatty liver disease (NAFLD). In this study, we introduced a chemiluminescence (CL) method combined with microarray technology and a simple fabrication procedure to obtain a highly sensitive SUA probe based on a mesoporous metal oxide nanomaterial. The high-throughput method was based on the generation of H<subscript>2</subscript>O<subscript>2</subscript> from SUA by immobilized uricase and its measurement by a CL reaction catalyzed by mesoporous metal oxide nanomaterials. The CL probe was designed for SUA The linear range of the uric acid concentration was 0.6-9 μM and the detection limit was 0.1 μM. In comparison with the other SUA detection techniques, this method has the advantages of a low detection limit, high sensitivity and simplicity. A new sensitive high-throughput approach was obtained for the determination of SUA. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15227235
- Volume :
- 32
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Luminescence: Journal of Biological & Chemical Luminescence
- Publication Type :
- Academic Journal
- Accession number :
- 123996778
- Full Text :
- https://doi.org/10.1002/bio.3243