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A reagentless nitric oxide biosensor based on haemoglobin/polyethyleneimine film.
- Source :
- Biotechnology & Applied Biochemistry; Oct2003, Vol. 38 Issue 2, p119-122, 4p
- Publication Year :
- 2003
-
Abstract
- An unmediated NO biosensor was prepared by co-immobilizing haemoglobin with polyethyleneimine at a pyrolytic graphite electrode surface. Haemoglobin exhibits direct electrochemistry in the membrane, and this modified electrode shows high stability and catalytic activity towards the reduction of NO. A linear relationship between the catalytic current and the concentration of NO was observed in the concentration ranges 1.0×10<superscript>-7</superscript>8.0×10<superscript>-6</superscript> M and 1.0×10<superscript>-5</superscript>2.5×10<superscript>-5</superscript> M, with a detection limit of 4.0×10<superscript>-8</superscript> M. [ABSTRACT FROM AUTHOR]
- Subjects :
- BIOSENSORS
HEMOGLOBINS
GRAPHITE
ELECTRODES
ELECTROCHEMISTRY
CELL membranes
Subjects
Details
- Language :
- English
- ISSN :
- 08854513
- Volume :
- 38
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Biotechnology & Applied Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12409854