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Simultaneous Analysis of Hydrophilic and Lipophilic Compounds in Salvia miltiorrhiza by Double-Development HPTLC and Scanning Densitometry.
- Source :
- Journal of Planar Chromatography - Modern TLC; Jun2011, Vol. 24 Issue 3, p257-263, 7p
- Publication Year :
- 2011
-
Abstract
- A double-development TLC method has been developed for simultaneous qualitative and quantitative analysis of hydrophilic and lipophilic constituents of Salvia miltiorrhiza (Danshen). The optimized mobile phases dichloromethane-ethyl acetate-formic acid 22:24:10 (v/v) and petroleum ether-ethyl acetate-cyclohexane 25:11:14 (v/v) were used for the double development on nano-silica gel 60F<subscript>254</subscript> plates. Their characteristic TLC profiles were observed under UV light at 254 and 365 nm and the bands were then revealed by reaction with 5% H<subscript>2</subscript>SO<subscript>4</subscript> in EtOH. Quantification of twelve compounds was achieved by densitometry at 260 or 290 nm, with reference at 400 nm. Linearity was quite good (R² > 0.99) within the ranges tested. This method could be used for quality control of Danshen. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09334173
- Volume :
- 24
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of Planar Chromatography - Modern TLC
- Publication Type :
- Academic Journal
- Accession number :
- 124172546
- Full Text :
- https://doi.org/10.1556/JPC.24.2011.3.16