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The Nodulin 26-like intrinsic membrane protein OsNIP3;2 is involved in arsenite uptake by lateral roots in rice.
- Source :
- Journal of Experimental Botany; 5/17/2017, Vol. 68 Issue 11, p3007-3016, 10p
- Publication Year :
- 2017
-
Abstract
- Previous studies have shown that the Nodulin 26-like intrinsic membrane protein (NIP) Lsi1 (OsNIP2;1) is involved in arsenite [As(III)] uptake in rice (Oryza sativa). However, the role of other rice NIPs in As(III) accumulation in planta remains unknown. In the present study, we investigated the role OsNIP3;2 in As(III) uptake in rice. When expressed in Xenopus laevis oocytes, OsNIP3;2 showed a high transport activity for As(III). Quantitative real-time RT-PCR showed that the expression of OsNIP3;2 was suppressed by 5 pM As(III), but enhanced by 20 and 100 pM As(III). Transgenic rice plants expressing OsNIP3;2pro-GUS showed that the gene was predominantly expressed in the lateral roots and the stele region of the primary roots. Transient expression of OsNIP3;2:GFP fusion protein in rice protoplasts showed that the protein was localized in the plasma membrane. Knockout of OsNIP3;2 significantly decreased As concentration in the roots, but had little effect on shoot As concentration. Synchrotron microfocus X-ray fluorescence showed decreased As accumulation in the stele of the lateral roots in the mutants compared with wild-type. Our results indicate that OsNIP3;2 is involved in As(III) uptake by lateral roots, but its contribution to As accumulation in the shoots is limited. [ABSTRACT FROM AUTHOR]
- Subjects :
- NODULINS
MEMBRANE proteins
ARSENITES
RICE
X-ray fluorescence
Subjects
Details
- Language :
- English
- ISSN :
- 00220957
- Volume :
- 68
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Journal of Experimental Botany
- Publication Type :
- Academic Journal
- Accession number :
- 124210579
- Full Text :
- https://doi.org/10.1093/jxb/erx165