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Quantification of plasmid loss in Escherichia coli cells by use of flow cytometry
- Source :
- FEMS Microbiology Letters; Mar2004, Vol. 232 Issue 1, p45, 5p
- Publication Year :
- 2004
-
Abstract
- A method was developed to study plasmid stability in Escherichia coli cells, which utilised the high speed analysis properties of flow cytometry. To discriminate between plasmid-harbouring cells and plasmid-free cells a plasmid-encoded Lac repressor protein was used to regulate the expression of a chromosomally inserted green fluorescent protein gene in the host cells. Flow cytometric analysis enabled detection and quantification of plasmid-free cells due to their green fluorescent phenotype. The reported system offers real-time analysis in combination with a very low detection level of plasmid loss in bacterial populations. This could be useful in future investigations of plasmid stability and population selection in bacterial communities. [Copyright &y& Elsevier]
- Subjects :
- CELLS
ENTEROBACTERIACEAE
ESCHERICHIA coli
GUMS & resins
Subjects
Details
- Language :
- English
- ISSN :
- 03781097
- Volume :
- 232
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- FEMS Microbiology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 12435412
- Full Text :
- https://doi.org/10.1016/S0378-1097(04)00015-1