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Isolation and characterisation of microsatellites for Xiphinema index using degenerate oligonucleotide primed PCR.

Isolation and characterisation of microsatellites for Xiphinema index using degenerate oligonucleotide primed PCR.

Authors :
Yu He
Hongmei Li
Brown, Derek J. F.
Lamberti, Franco
Moens, Maurice
Source :
Nematology; 2003, Vol. 5 Issue 6, p809-819, 11p, 2 Black and White Photographs, 2 Diagrams, 4 Charts, 1 Graph
Publication Year :
2003

Abstract

A short insert genomic library for Xiphinema index, the natural vector of Grapevine Fanleaf Virus, was constructed from degenerate oligonucleotide primed PCR (DOP-PCR) products. The genomic library was screened for (CA)[sub n] microsatellites. Screening of 6200 colonies and comparison of the sequencing results revealed seven (CA)[sub n] containing microsatellites, coded here as XIMSL1, XIMSL2, XIMSL3, XIMSL4, XIMSL5, XIMSL6 and XIMSS1. XIMSL prefixed microsatellites were followed by the motif of the same long interspersed element. Microsatellite XIMSS1 has some similarity to the short interspersed element. Except for XIMSL1, all microsatellites were proven to be effective diagnostic tools at species level. Genetic diversity between and within populations was also evaluated for each microsatellite. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13885545
Volume :
5
Issue :
6
Database :
Complementary Index
Journal :
Nematology
Publication Type :
Academic Journal
Accession number :
12461467
Full Text :
https://doi.org/10.1163/156854103773040718