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Hen egg yolk phosvitin stimulates osteoblast differentiation in the absence of ascorbic acid.
- Source :
- Journal of the Science of Food & Agriculture; Oct2017, Vol. 97 Issue 13, p4532-4538, 7p
- Publication Year :
- 2017
-
Abstract
- BACKGROUND Egg yolk phosvitin, one of the most highly phosphorylated extracellular matrix proteins known in nature, has a strong calcium binding and reducing capacity. Here, we investigated the effects of phosvitin on osteoblast differentiation and osteogenic gene expression in cultured mouse osteoblastic MC3T3-E1 cells by using alkaline phosphatase activity analysis, alizarin red S staining and real-time PCR assay. RESULTS Alkaline phosphatase activity and alizarin red S staining analyses demonstrated no significant difference between differentiating MC3T3-E1 cells cultured in the presence of phosvitin and those cultured in the presence of ascorbic acid after 21 days of differentiation. Our real-time PCR assay also indicated the two groups were similar in the expression of the osteogenic gene markers, collagen type I, osteocalcin, runt-related transcription factor 2, and bone morphogenetic protein-2. CONCLUSION Our findings indicate that phosvitin plays a similar role to that of ascorbic acid in osteoblast differentiation and mineralisation. © 2017 Society of Chemical Industry [ABSTRACT FROM AUTHOR]
- Subjects :
- EGG yolk
OSTEOBLASTS
VITAMIN C
Subjects
Details
- Language :
- English
- ISSN :
- 00225142
- Volume :
- 97
- Issue :
- 13
- Database :
- Complementary Index
- Journal :
- Journal of the Science of Food & Agriculture
- Publication Type :
- Academic Journal
- Accession number :
- 125110067
- Full Text :
- https://doi.org/10.1002/jsfa.8320