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Pre- and Post-Transfusion Alloimmunization in Dogs Characterized by 2 Antiglobulin-Enhanced Cross-match Tests.
- Source :
- Journal of Veterinary Internal Medicine; Sep/Oct2017, Vol. 31 Issue 5, p1420-1429, 10p
- Publication Year :
- 2017
-
Abstract
- Background When dogs are transfused, blood compatibility testing varies widely but may include dog erythrocyte antigen ( DEA) 1 typing and rarely cross-matching. Objectives Prospective study to examine naturally occurring alloantibodies against red blood cells ( RBCs) and alloimmunization by transfusion using 2 antiglobulin-enhanced cross-match tests. Animals Eighty client-owned anemic, 72 donor, and 7 control dogs. Methods All dogs were typed for DEA 1 and some also for DEA 4 and DEA 7. Major cross-match tests with canine antiglobulin-enhanced immunochromatographic strip and gel columns were performed 26-129 days post-transfusion (median, 39 days); some dogs had an additional early evaluation 11-22 days post-transfusion (median, 16 days). Plasma from alloimmunized recipients was cross-matched against RBCs from 34 donor and control dogs. Results The 2 cross-match methods gave entirely concordant results. All 126 pretransfusion cross-match results for the 80 anemic recipients were compatible, but 54 dogs died or were lost to follow up. Among the 26 recipients with follow-up, 1 dog accidently received DEA 1-mismatched blood and became cross-match-incompatible post-transfusion. Eleven of the 25 DEA 1-matched recipients (44%) became incompatible against other RBC antigens. No naturally occurring anti- DEA 7 alloantibodies were detected in DEA 7− dogs. Conclusions and clinical importance The antiglobulin-enhanced immunochromatographic strip cross-match and laboratory gel column techniques identified no naturally occurring alloantibodies against RBC antigens, but a high degree of post-transfusion alloimmunization in dogs. Cross-matching is warranted in any dog that has been previously transfused independent of initial DEA 1 typing and cross-matching results before the first transfusion event. [ABSTRACT FROM AUTHOR]
- Subjects :
- BLOOD coagulation
IMMUNOGLOBULINS
ANTIGENS
ERYTHROCYTES
BLOOD plasma
IMMUNITY
Subjects
Details
- Language :
- English
- ISSN :
- 08916640
- Volume :
- 31
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Journal of Veterinary Internal Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 125145758
- Full Text :
- https://doi.org/10.1111/jvim.14801