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Halorubrum salsamenti sp. nov., a Novel Halophilic Archaeon Isolated from a Brine of Salt Mine.
- Source :
- Current Microbiology; Nov2017, Vol. 74 Issue 11, p1358-1364, 7p
- Publication Year :
- 2017
-
Abstract
- A non-motile, spherical or oval extremely halophilic archaeon, strain Y69, was isolated from a brine of the Yunnan salt mine, China. Colonies on JCM 168 agar plate were round (1-2 mm in diameter), moist, and orange-pigmented. Phylogenetic analysis of the almost-complete 16S rRNA gene sequence showed that the isolate belonged to the species of the genus Halorubrum, with a close relationship to Halorubrum aidingense 31-hong (98.5%), Halorubrum lacusprofundi ATCC 49239 (98.2%), and Halorubrum kocurii BG-1 (98.0%). The major polar lipids of strain Y69 were phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl diether. Strain Y69 grew in 15-30% (w/v) NaCl. The temperature and pH ranges for growth were 25-50 °C and 6.5-9.0, respectively. Optimal growth occurred at 20% (w/v) NaCl, 42 °C, and pH 8.0. Mg was required for growth. The DNA G+C content was determined to be 65.1 mol% by the thermal denaturation method. DNA-DNA hybridization values between strain Y69 and the closely related species were lower than 70%. Based on the data presented in this study, strain Y69 represents a novel species for which the name Halorubrum salsamenti sp. nov. is proposed. The type of the strain is Y69 (=CGMCC 1.15455 = JCM 31270). [ABSTRACT FROM AUTHOR]
- Subjects :
- HALOPHILIC animals
NUCLEIC acid hybridization
PHENOTYPES
CHEMOTAXONOMY
SALT mining
Subjects
Details
- Language :
- English
- ISSN :
- 03438651
- Volume :
- 74
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Current Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 125695138
- Full Text :
- https://doi.org/10.1007/s00284-017-1325-8