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Differentiation of the serotype b and species-specific antigens of <em>Actinobacillus actinomycetemcomitans</em> recognized by monoclonal antibodies.
- Source :
- Oral Microbiology & Immunology; Aug1996, Vol. 11 Issue 4, p209-219, 11p
- Publication Year :
- 1996
-
Abstract
- The serotype b antigens have been reported to be associated with lipopolysaccharide. Using murine monoclonal antibodies specific for either a serotype b antigen or the <em>Actinobacillus actinomycetemcomitans</em> species, the relationship of the two epitopes to lipopolysaccharide was determined. Both the species-specific and serotype b-specific monoclonal antibodies bound to whole cells, vesicles and conventionally isolated lipopolysaccharide and polysaccharide material derived from <em>A. actinomycetemcomitans</em> culture supernatants. Serotype b-specific monoclonal antibodies bound to the polysaccharide of acid-hydrolyzed lipopolysaccharide. Species-specific monoclonal antibodies bound to both the polysaccharide and the lipid A fraction of lipopolysaccharide after acid hydrolysis. Polymyxin b partially inhibited the binding of the species-specific monoclonal antibodies to lipopolysaceharide and had no effect on the binding of the serotype b-specific monoclonal antibodies to lipopolysaccharide. Lipopolysaccharide from whole bacteria and polysaccharide material isolated from culture supernatants were separated by gel filtration chromatography in deoxycholate into fractions that contained serotype b antigen, both serotype b and species-specific antigens, or species-specific antigen. SDS-polyacrylamide gel electrophoresis and Western blotting analysis of the fractions revealed that the serotype b antigen was on a high-molecular-weight polysaccharide material. The species-specific antigen was on a ladder of lower-molecular-weight polysaccharides identical to the blot pattern of hpopolysaccharide molecules separated by polyacrylamide gel electrophoresis and stained with silver stain. Chemical analysis of the polysaccharide containing serotype b antigen revealed 85% ribose, 11% glucose, and no lipid. Chemical content of the species-specific antigenic material revealed a composition typical of lipopolysaccharide. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09020055
- Volume :
- 11
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Oral Microbiology & Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12596314
- Full Text :
- https://doi.org/10.1111/j.1399-302X.1996.tb00172.x