Back to Search
Start Over
Aspartyl proteases from the intestinal brush border of Haemonchus contortus as protective antigens for sheep.
- Source :
- Parasite Immunology; Nov2003, Vol. 25 Issue 11/12, p521-530, 10p
- Publication Year :
- 2003
-
Abstract
- A novel pepsin-like aspartyl protease was identified as a component of Haemonchus galactose-containing glycoprotein (H-gal-GP), which is an integral membrane glycoprotein complex located on the intestinal cells of Haemonchus contortus, and a highly protective antigen for sheep. This molecule, designated HcPEP2, showed 50% sequence identity with a previously described aspartyl protease from H-gal-GP known as HcPEP1. Fractions of H-gal-GP, either containing both HcPEP1 and 2 or other lower molecular weight components of the complex, were evaluated as protective antigens in immunization – challenge trials in sheep. When separated from the rest of the complex by gel filtration in 8  m urea, the HcPEP1 and 2 fraction significantly reduced H. contortus egg counts by 48% and worm numbers by 36%, but the lower molecular weight components were not significantly protective. However, the HcPEP1 and 2 fraction did not protect if electro-eluted from SDS-dissociated H-gal-GP, nor did bacterially expressed recombinant HcPEP1, suggesting that conformational epitopes are important for inducing immunity. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROTEOLYTIC enzymes
HAEMONCHUS contortus
GLYCOPROTEINS
ANTIGENS
IMMUNITY
IMMUNIZATION
Subjects
Details
- Language :
- English
- ISSN :
- 01419838
- Volume :
- 25
- Issue :
- 11/12
- Database :
- Complementary Index
- Journal :
- Parasite Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12606483
- Full Text :
- https://doi.org/10.1111/j.0141-9838.2004.00667.x