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A Strategy for Specific Fluorescence Imaging of Monoamine Oxidase A in Living Cells.
- Source :
- Angewandte Chemie; 11/27/2017, Vol. 129 Issue 48, p15521-15525, 5p
- Publication Year :
- 2017
-
Abstract
- Monoamine oxidase (MAO) has two isoforms, MAO-A and MAO-B, which show different functions, and thus selective fluorescence imaging is important for biological studies. Currently, however, specific detection of MAO-A remains a great challenge. Herein, we report a new strategy for specific imaging of MAO-A through the design of fluorogenic probes combining the characteristic structure of an inhibitor of the target enzyme along with propylamine as a recognition moiety. The high specificity of our representative probe is demonstrated by imaging MAO-A in different live cells such as SH-SY5Y (high levels of MAO-A) and HepG2 (high levels of MAO-B), and further validated by western blot analyses. The superior specificity of the probe may enable the accurate detection of MAO-A in complex biosystems. Importantly, the use of the characteristic structure of an inhibitor, as demonstrated in this work, may serve as a general strategy to design specific recognition moieties for fluorogenic probes for enzymes. [ABSTRACT FROM AUTHOR]
- Subjects :
- MONOAMINE oxidase
IMAGING systems
BIOSENSORS
FLUORESCENT probes
PROPYLAMINE
Subjects
Details
- Language :
- English
- ISSN :
- 00448249
- Volume :
- 129
- Issue :
- 48
- Database :
- Complementary Index
- Journal :
- Angewandte Chemie
- Publication Type :
- Academic Journal
- Accession number :
- 126405123
- Full Text :
- https://doi.org/10.1002/ange.201708428